Protocols Skeletal preparation of elasmobranch embryos and hatchlings Wholemount chromogenic mRNA in situ hybridisation Chromogenic mRNA in situ hybridisation on paraffin sections in situ hybridisation by chain reaction (HCR) on paraffin sections (after Choi et al.) Immunofluorescence/in situ hybridisation by chain reaction (HCR) on paraffin sections (after Schwartzkopf et al.) Standard immunofluorescence on paraffin sections Modified Masson’s trichrome staining (after Witten and Hall)
